Organelle DNA Replication Genes Enable Tomato Fertility Recovery 🍅⚡

 Fertility restoration in cytoplasmic male sterile (CMS) tomato represents a major breakthrough in plant biotechnology 🌱🍅. CMS is widely used in hybrid breeding but limits pollen production, affecting seed yield. By targeting nuclear-encoded organellar DNA replication genes, researchers have uncovered a precise genetic route to reverse male sterility 🧬✨.

In this study, knockout of DNA polymerase I or Topoisomerase I successfully restored fertility in CMS tomato plants 🔬🌿. These genes play a critical role in mitochondrial and chloroplast DNA replication, and their disruption alters abnormal organellar gene expression responsible for sterility ⚙️🧪. The result is normal pollen development and improved reproductive performance 🌸.

This discovery opens new possibilities for hybrid seed production and crop improvement 🚜🌍. Instead of relying on traditional fertility restorer genes, breeders can now use targeted genome editing to control fertility with high precision 🎯🧬. Such strategies promise more efficient, sustainable, and scalable tomato breeding for future agriculture 🍅🌞.

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